IMB Microscopy


Current Status:  Fully Operational


Resource Links:


Room 6.018 - Olympus IX81 Inverted Microscope Stand with Hamamatsu Orca CCD camera

Inverted widefield microscope with Xenon lightsource, high speed filter wheels and CCD camera. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.

Features Include:

  • Fully motorised X-Y-Z stage
  • CO2 and temperature incubation for live cells and tissue
  • Tiled imaging
  • Multi-position imaging
  •  

Transmitted Light:  Olympus 100W Halogen Lamp

Reflected Light:  Olympus MT20 Xenon White Lightsource

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD 0.45 54 Open PhL Ph1 Ph2 DIC I DIC II

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 10x 0.4 Dry - DIC   M27
2 Plan Apochromat 20x 0.75 Dry - DIC   M27
3 Plan Apochromat 40x 1.0 Oil - DIC   M27
4 Plan Apochromat 60x 1.35 Oil - DIC   M27

 

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 GFP/RFP S500/20 dual band dual band GFP, RFP, mCherry
2 CFP/YFP S430/25

dual band

dual band

CFP, YFP
3   S565/55      
4   S470/30      

 

Computer:

Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU

1TB Raid Array

30" LCD Monitor

 

Software: 

Cell^R

 

Accessories:

Incubation chamber with CO2 and Temperature control

 

 

Contact

For General Enquiries

Microscopes@imb.uq.ed.au

 

Microscopy Staff

Microscopy Facility Manager

James Springfield

j.springfield@uq.edu.au

07 33462390

 

Microscopy Officers

Nicholas Condon

n.condon@uq.edu.au

Ph: 07 33462042

Mob: 0400909510