IMB Microscopy


Current Status:  Fully Operational


Resource Links:


Room 6.025c - Nikon Ti-E Inverted Microscope Stand with Hamamatsu Flash 4.0 sCMOS camera

Inverted widefield microscope with Lumencor 7 line LED lightsource, high speed filter wheels and CMOS camera. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.

Features Include:

  • Fully motorised X-Y-Z stage
  • Piezo Z-drive for rapid Z-stacks
  • CO2 and temperature incubation for live cells and tissue
  • Tiled imaging
  • Multi-position imaging
  •  

Transmitted Light:  Sutter 100W LED Lamp

Reflected Light:  Lumencor Spectra LED Lightsource

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD 0.45 54 - - - - - -

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 4x 0.2 Dry - -   M27
2 Plan Apochromat 10x 0.45 Dry 4 DIC   M27
3 Plan Apochromat 20x 0.75 Dry 1 DIC   M27
4 Plan Apochromat 40x 0.95 Dry 0.25 DIC   M27
5 S Fluor 40x 1.30 Oil 0.22 DIC   M27
6 Plan Apochromat 60x 1.4 Oil 0.13 DIC   M27

 

Fluorescent Filters:

Excitation Dichroic Emission
390/18 410/504/582/669 440/521/607/700
438/24 462/523 440/40
485/20 505/606 479/40
513/17 436/514/604 525/30
560/25 488LP 542/27
585/29   631/36
650/13   607/36
    692/40
    488LP

 

LEDs:

Designation

Wavelength

Power

BFP Power*

Suitable Dyes

390

390nm

-

-

DAPI / Hoechst

440

440nm

-

-

CFP

485

485nm

-

-

GFP / A488

514

514nm

-

-

YFP

563

563nm

-

-

mCherry / Cy3

594

594nm

-

-

A594

640

640nm

-

-

Cy5 / A647

*BFP = Back Focal Plane of Objective

 

Computer:

Dell PC - 3GHz Xeon Processor - 32GB RAM - 1GB nVidia Quadro GPU

3TB Raid Array

30" LCD Monitor

 

Software: 

NIS Elements 4.3

 

Accessories:

Incubation chamber with CO2 and Temperature control

 

 

 

Contact

For General Enquiries

Microscopes@imb.uq.ed.au

 

Microscopy Staff

Microscopy Facility Manager

James Springfield

j.springfield@uq.edu.au

07 33462390

 

Microscopy Officers

Nicholas Condon

n.condon@uq.edu.au

Ph: 07 33462042

Mob: 0400909510