IMB Microscopy

Current Status:  Fully Operational


Resource Links:


Room 6.029a - Leica DMi8 Inverted Microscope Stand with SP8 Galvo and Resonant Confocal Scanners, White Light Laser (WLL), STED 3X super-resolution, and FLIM/FRET

Inverted point-scanning laser confocal microscope with spectral, super resolution and FLIM/FRET detection. Tuneable 470nm - 670nm WLL, 2 x PMT and 2 x HyD detectors. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.

Features Include:

  • Fully motorised X-Y-Z stage
  • High-sensitivity HyD detectors 
  • CO2 and temperature incubation for live cells and tissue
  • Tiled imaging
  • Multi-position imaging
  • Spectral imaging
  • Super-Resolution Imaging with 592nm, 660nm and 775nm STED lasers
  • Fast Resonant Imaging
  • FLIM/FRET Imaging with WLL and 440nm pulsed lasers

 

Transmitted Light:  Leica LED Lamp

Reflected Light:  Leica EL6000 120W White Light Lamp

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD 0.55 28 - K3 K6 K7 - -

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 HC Plan Apochromat 10x 0.4 Dry 2.2 DIC    
2 HC Plan Apochromat 20x 0.75 Multi 0.67 DIC    
3 HC Plan Apochromat 40x 1.1 Water 0.65 DIC    
                 
5

HC Plan Apochromat

93x 1.30 Glyc 0.3 DIC    
6 HC Plan Apochromat 100x 1.40 Oil 0.13 DIC    

 

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1

DAPI

350/50

 

460/50

DAPI, Hoechst

2

GFP

470/40

 

525/50

EGFP, A488

3

YFP

500/20

 

535/30

YFP

4 Texas Red 560/40   630/75 Texas Red, mCherry
           

 

Lasers:

Laser

Wavelength

Laser Power

BFP Power*

Suitable Dyes

405

405nm

50mW

-

DAPI / Hoechst

440 pulsed

440nm

-

-

CFP FLIM/FRET

442

442nm 40mW - CFP
WLL

470nm-670nm

1.5mW

-

Visible Dyes

92 STED

592nm

1.5W

-

Star 488, Oregeon Green

660 STED

660nm

1.5W

-

Star 470sx, A546

775 STED

775nm

1.5W

-

SiR

*BFP = Back Focal Plane of Objective

 

Computer:

HP Z640 PC - 3GHz Xeon Processor - 64GB RAM - 24GB nVidia Quadro M6000 GPU

3TB Raid Array

2 x 30" LCD Monitor

 

Software: 

Leica LASX Software

Co-localisation, 3D visualisation, FRAP, FRET, SymphoTime FLIM

 

Accessories:

Incubation chamber with CO2 and Temperature control

 

 

 

Contact

For General Enquiries

Microscopes@imb.uq.ed.au

 

Microscopy Staff

Microscopy Facility Manager

James Springfield

j.springfield@uq.edu.au

07 33462390

 

Microscopy Officers

Nicholas Condon

n.condon@uq.edu.au

Ph: 07 33462042

Mob: 0400909510