IMB Microscopy


Current Status:  Fully Operational


Resource Links:


Room 3.011 - Zeiss AxioImage M2.m Microscope Stand with Apotome2 and Axiocam 506 camera

Inverted widefield microscope with Mercury lightsource and CCD camera. Suited for imaging fixed and live fluorescent samples.

Features Include:

Motorised X-Y-Z stage, Apotome2

Transmitted Light:  LED illumination

Reflected Light:  HXP 120c White Lightsource

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Motorised Universal 0.9     DIC I DIC II DIC III    

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 EC Plan NeoFluar 5x 0.16 Dry 18.5 DIC   M27
2 Plan Apochromat 10x 0.30 Dry 2 DIC   M27
3 Plan Apochromat 20x 0.80 Dry 0.55 DIC   M27
4 Plan Neofluar 40x 1.30 Oil 0.21 DIC   M27
5 Plan Apochromat 63x 1.40 Oil 0.14 DIC   M27
6 Plan Apochromat 100x 1.40 Oil 0.17 DIC   M27

 

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI 49 365 395 445/50 DAPI, Hoechst
2 FITC 44 475/40 500 530/50 EGFP, Alexa Fluor 488
3 Cy3 43 545/25 570 605/70 Cy3
4 Texas Red 45 560/40 585 630/75 Tx Red, mCherry, A568
5 Cy5 50 640/30 660 690/50 Cy5, A647
6 Empty - - - Brightfield

 

Computer:

HP Z820 PC - 3GHz Xeon Processor -64GB RAM - 1GB ATI Firepro V5800 GPU

1TB Raid Array

30" LCD Monitor

 

Software: 

Zeiss Zen 2 with Tiling and EDF modules

Accessories:

 

Contact

For General Enquiries

Microscopes@imb.uq.ed.au

 

Microscopy Staff

Microscopy Facility Manager

James Springfield

j.springfield@uq.edu.au

07 33462390

 

Microscopy Officers

Nicholas Condon

n.condon@uq.edu.au

Ph: 07 33462042

Mob: 0400909510