IMB Microscopy


Current Status:  Fully Operational


Resource Links:


Room 6.025a - Nikon Ti-U Inverted Microscope Stand with Nikon DS-Fi1 and Qi1 CCD cameras

Inverted widefield microscope with Mercury lightsource and CCD camera. Suited for imaging fixed and live fluorescent samples.

Features Include:

  • Manual X-Y-Z stage
  •  

Transmitted Light:  Nikon 100W Halogen Lamp

Reflected Light:  Nikon 100W Mercury White Lightsource

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD 0.45 54 Open PhL Ph1 Ph2 DIC I DIC II

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Fluor 4x 0.13 Dry - PhL DL M27
2 Plan Fluor 10x 0.30 Dry - Ph1 DL M27
3 Plan Fluor ELWD 20x 0.45 Dry - Ph1 ADL M27
4 Plan Fluor ELWD 40x 0.60 Dry - Ph2 ADL M27
5 Plan Fluor 100x 1.30 Oil - Ph3 DDL M27
6 - - - - - - - M27

 

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI - 5060C 377/50 409LP 447/60 DAPI, Hoechst
2 CFP - 2432C 438/24 458LP 483/32 ECFP, Cerulean
3 GFP-3000B 488/10 505LP 530/60 EGFP, Alexa Fluor 488
4 YFP-2427B 500/24 520LP 542/27 EYFP, mVenus
5 mCherry-40LP-A 562/40 593LP 593LP mCherry Alexa Fluor 568
6 Empty - - - Brightfield

 

Computer:

Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU

1TB Raid Array

30" LCD Monitor

 

Software: 

NIS Elements-BR, NIS Elements F

Accessories:

Motorised Z-Focus

Contact

For General Enquiries

Microscopes@imb.uq.ed.au

 

Microscopy Staff

Microscopy Facility Manager

James Springfield

j.springfield@uq.edu.au

07 33462390

 

Microscopy Officers

Nicholas Condon

n.condon@uq.edu.au

Ph: 07 33462042

Mob: 0400909510