IMB Microscopy


Current Status:  Fully Operational


Resource Links:


Room 6.019b - Zeiss Axiovert 200 Inverted Microscope Stand with LSM 710 Meta Confocal Scanner

Upright point-scanning laser confocal microscope with spectral detection  Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.

Features Include:

  • Fully motorised X-Y-Z stage
  • Tiled imaging
  • Multi-position imaging
  • Spectral imaging

 

Transmitted Light:  Zeiss 100W Halogen Lamp

Reflected Light:  Exfo Xcite 120W White Light Lamp

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
- - - - - - - - -

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 10x 0.45 Dry - -   M27
2 Plan Apochromat 20x 0.80 Dry - - - M27
3 Plan Apochromat 40x 1.3 Oil - -   M27
4 Plan Apochromat 63x 1.4 Oil - -   M27

 

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 Zeiss #63 HE 572/25 FT 590 629/62 mCherry, Texas Red
2 Zeiss #38 HE 470/40 FT 495 525/50 EGFP, Alexa Fluor 488
3 Zeiss #47 HE 436/25 FT 455 480/40 DAPI, Hoechst
4 - - - - -
5 - - - - -

 

Lasers:

Laser

Wavelength

Laser Power

BFP Power*

Suitable Dyes

405

405nm

-

-

DAPI / Hoechst

Argon Ion

458nm

- -

CFP

Argon Ion

488nm

- -

GFP / Alexa Fluor488

Argon Ion

514nm

- -

YFP

561

561nm

- -

mCherry / Cy3

633

633nm

- -

Cy5 / A647

*BFP = Back Focal Plane of Objective

 

Computer:

Fujitsu PC - 3GHz Xeon Processor - 32GB RAM - 1GB nVidia Quadro GPU

3TB Raid Array

30" LCD Monitor

 

Software: 

Zeiss Zen 2012 Black

 

Accessories:

Incubation chamber with CO2 and Temperature control

 

 

 

Contact

For General Enquiries

Microscopes@imb.uq.ed.au

 

Microscopy Staff

Microscopy Facility Manager

James Springfield

j.springfield@uq.edu.au

07 33462390

 

Microscopy Officers

Nicholas Condon

n.condon@uq.edu.au

Ph: 07 33462042

Mob: 0400909510