IMB Microscopy


Current Status:  Fully Operational


Resource Links:


Room 6.033 - Custom Inverted Microscope Stand with Hamamatsu Orca CCD camera

Inverted widefield microscope with Dual Mercury lightsources, high speed filter wheels and CCD camera. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.

Features Include:

  • Fully motorised X-Y-Z stage
  • CO2 and temperature incubation for live cells and tissue
  • Tiled imaging
  • Multi-position imaging
  •  

Transmitted Light: 10W LED Lamp

Reflected Light:  Olympus Mercury White Lightsource

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 4x 0.5 Dry 2 PhL ADL M27
2 Plan Apochromat 10x 0.75 Dry 0.5 Ph1   M27
3 Plan Apochromat 20x 0.75 Dry 0.4 Ph2 ADL M27
4 Plan Apochromat 40x 0.95 Dry 0.2 DIC I   M27

 

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI - 5060C 377/50 409LP 447/60 DAPI, Hoechst
2 CFP - 2432C 438/24 458LP 483/32 CFP, Cerulean
3 GFP-3000B 488/10 505LP 530/60 GFP, A488
4 mCherry LP 560/20 580LP 585LP mCherry, Cy3
5   - - -  
6   - - -  

 

Computer:

Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU

1TB Raid Array

30" LCD Monitor

 

Software: 

Pathway

 

Accessories:

Incubation chamber with CO2 and Temperature control

 

 

Contact

For General Enquiries

Microscopes@imb.uq.ed.au

 

Microscopy Staff

Microscopy Facility Manager

James Springfield

j.springfield@uq.edu.au

07 33462390

 

Microscopy Officers

Nicholas Condon

n.condon@uq.edu.au

Ph: 07 33462042

Mob: 0400909510